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Whole-cell patch clamp recordings Mice were anesthetized with isoflurane and decapitated around 10 AM, the brain was rapidly removed and immersed in a cold (4 C) and oxygenated cutting solution containing (mM): sucrose 220, KCl 2.5, NaH 2 PO 4 1.23, NaHCO 3 26, CaCl 2 1, MgCl 2 6 and glucose 10 (pH 7.3 with NaOH)
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