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Glycerol was added to a final concentration of 10% as well as turbonuclease (5 l) and a protease inhibitor cocktail tablet (Roche)
First, the sulfonation reaction saturates and that allows more NAPQI to accumulate ([7, 24]) and second, the increased amount of NAPQI exhausts the liver stores of reduced glutathione (GSH)[6] as well as the livers capacity to synthesize new GSH
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