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Key Strategies: Use multiple enzymes (e.g., Trypsin + Glu-C) for comprehensive cleavage Optimize pH, temperature, and incubation time Maintain proper enzyme-to-substrate ratios Outcome: Reduces missed cleavages Prevents overdigestion Enhances overall mapping accuracy 2
Other Medications Weight loss pills like phentermine or orlistat can deliver short-term results, but they often carry more side effects and dont always address the metabolic drivers of weight gain
Patients with a personal or family history of medullary thyroid carcinoma or multiple endocrine neoplasia type 2 should not use GLP-1 agonists, as animal studies suggested potential thyroid C-cell effects (though human data remain reassuring)
The hormonal imbalances caused by obesityincluding insulin resistance, elevated androgens, and disrupted leptin signallingcan significantly affect the hypothalamic-pituitary-ovarian axis
