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After that, cells were washed with ice-cold PBS and lysed by 50 L lysis buffer (PBS supplemented with 20 mM Tris-HCl and 1% (v/v) Triton X-100, pH 7.4)
Human glucagon-like peptides 1 and 2 activate rat brain adenylate cyclase
Store at room temperature 68-77F (20-25C)
Setting expectations appropriately based on individual circumstances helps maintain adherence through the time needed for full benefit
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