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INS-1 832/3 cells lacking endogenous GLP-1R after deletion by CRISPR/Cas9 (Naylor et al., 2016) (a gift from Dr Jacqueline Naylor, MedImmune) were used to generate a polyclonal population expressing human SNAP-GLP-1R by G418 selection, and were maintained as for wild-type INS-1 832/3 cells, with the addition of G418 (1 mg/ml)

This approach common to GLP-1 and related receptor agonist therapies is designed to minimise gastrointestinal side effects such as nausea, vomiting, and diarrhoea, which are among the most frequently reported adverse events with this class of medication

Robert Kushner: Yeah, compounding is something that the FDA approves when a medication needs to be modified for a particular individual

Licensing deals, co-development partnerships, and technology acquisitions are accelerating innovation cycles