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Lipofectamine3000 reagent was used for the plasmid transfection, whereas Lipofectamine RNAiMAX was used for the transfection of siRNAs, all these transfections were performed according to the manufacturers protocol, and the cells were harvested at 48 h post-transfection in case of plasmid-mediated overexpression and at 72 h for siRNAs-mediated gene knockdown
Primary coronary angiography exhibiting normal vessels with new stenosis equal to or exceeding 30%
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