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showed that intracellular glutamate accumulation following cyst(e)ine depletion or system x C - inhibition is a key contributor to the induction of ferroptosis, and glutamate toxicity can be alleviated by converting excess glutamate to -glutamyl-di- or tripeptides via promiscuous enzymatic activities of glutamate-cysteine ligase catalytic subunit (GCLC) 12
After washing, horseradish peroxidase-linked secondary antibodies were applied for 30 min at room temperature, and chemiluminescence was developed using an ECL substrate (Thermo Fisher Scientific)
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When results fail, the peptide is rarely the problemit's the variables introduced before the first dose was ever administered
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