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Cell viability during fluorescence-activated cell sorting analysis was determined using the LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (L10119, Invitrogen)

Subsequently, they used Western blot analysis and RNA sequencing (RNA-seq) to demonstrate that the expression of GPX4 was declining in the early (MI 1 day) and middle (MI 1 week) stages of MI

Exploratory and hypothesis-generating metabolomics approaches suggest a trend toward a deeper alteration of the antioxidant system in severe IPTB, and NACs role in H 2 S and NO metabolism