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Discussion The present study showed that 3MST produces persulfurated species such as Cys-SSH and GSSH together with H 2 S 2 and H 2 S 3 as signaling molecules, and increases the levels of persulfurated proteins in a similar manner (Figs 1, 37, and Supplementary Figs S4 and S5) 14, 26, 40

However, no difference was found between the two groups when comparing changes in total glutathione levels (responders p = 0.05, non-responders p = 0.06) (Table 4)

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Free radicals can damage skin cells and contribute to aging, and glutathiones antioxidant properties may help counteract these effects