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Samples were analyzed (0.5 L injection volume) via an ALEX-CIS GCTOF mass spectrometry at the UC Davis Genome Centre Core Facilities, using a Rtx5Sil-MS column (Restek Corporation, USA, 30 m length x 0.25 mm internal diameter with 0.25 m film made of 95% dimethyl/5% diphenyl polysiloxane) and gas flow rate of 1 mL/min helium flow
Our study indicates that upregulation of p53-pS15 and p16 maintains a persistent senescent microenvironment to promote cell cycle arrest and apoptosis resistance in keloid fibroblasts
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