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The vector, pET 15b (Figure 1A-1), was constructed by inserting a flexible spacer sequence, a hexa-histadine tag and thrombin cleavable sequence (Figure 1B, top)

Type 2 diabetes remission 2 years post Roux-en-Y gastric bypass and sleeve gastrectomy: the role of the weight loss and comparison of DiaRem and DiaBetter scores

That observation became the foundation for one of the most extensively cited copper-peptide research lines in tissue-biology literature, and its the reason GHK-Cu is still classified in research today as an endogenous signaling peptide rather than a synthetic compound designed from scratch