After a further centrifugation (200 x g, 8 min, 20C, Universal 320, Andreas Hettich GmbH, Tuttlingen, Germany), PBMC were resuspended in RPMI medium supplemented with fetal bovine serum, HEPES buffer, L-glutamine, and -mercaptoethanol (all purchased from Biochrom AG, Berlin, Germany)
They mimic the hormone GLP-1, which slows down stomach emptying to reduce hunger and control blood sugar
This makes NAC a popular supplement among athletes and fitness enthusiasts looking to gain a competitive edge
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