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The protein was expressed in Sf9 insect cells (Invitrogen) using the Bac-to-Bac baculovirus expression system (ThermoFisher)

Apart from its participation in fat metabolism, the action of L-carnitine also includes: providing acetyl groups for the production of the neurotransmitter acetylcholine, reducing the production of lactic acid, thereby increasing the ability of muscles to perform, metabolism of branched-chain amino acids (BCAA), carbohydrate metabolism - high levels of L-carnitine in muscles reduce the use of glycogen as an energy material (and increase the use of fat), which is particularly important during prolonged exercise, and inhibits the formation of fat tissue, antioxidant activity, chelating certain metal ions, facilitating their transport and removal of harmful excess from the body