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The reaction mixture consisted of 1.53 mL phosphate buffer (0.05 M, pH 7.0), 0.1 mL of ethylene diaminetetra acetic acid (1 mM), 0.1 mL of sodium azide (1 mM), 0.1 mL of glutathione (1 mM), 0.1 mL of NADPH (0.2 mM), 0.01 mL of hydrogen peroxide (0.25 mM) and 0.02 mL of PMS in a final volume of 3 mL

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