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Cox, Lehninger Principles of Biochemistry, 7th Edition, W

The Ni-NTA resin was packed into a glass column and washed with 25 column volumes of 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, followed by elution using the same buffer supplemented with 250 mM imidazole and 3 mM CaCl 2

Although selenium deficiency, especially if severe, can impact thyroid health, it should be acknowledged that the role of selenium supplementation as a preventive or therapeutic strategy in thyroid diseases is highly controversial