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Mammals usually rely solely on extracellular uptake as the primary source of Cys, but some mammals also use cystathionine -lyase (CGL)-mediated cystathionine-cleavage in the transsulfuration pathway as a surrogate Cys source if System X c is blocked (Shimada and Stockwell, 2016)
The procedure was as follows: equilibration of the affinity gel as a batch using a glass filter funnel with binding/washing buffer consisting of 10 mM TrisHCl pH 7.8, 0.2 M NaCl, 0.2 mM dithiothreitol (DTT) and 1 mM EDTA
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10.1021/ACSAMI.4C01265 70 HasanA
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