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The lysate was subsequently clarified by centrifugation, bound in-batch to glutathione sepharose 4B resin (Cytiva), washed extensively with sonication buffer, and subjected to on-resin TEV protease cleavage in 25 mM Tris pH 7.5, 75 mM NaCl overnight at 4 C
Relative intensities of the serum glutathione levels in young ( n = 4) and old ( n = 4) mice as determined by LC-MS analysis ( B )
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