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Additional enrichment analyses were conducted for cell-type markers (via Fishers exact test) and Gene Ontology (GO) terms using the clusterProfiler R package

Neuroendocrine experiments use pituitary cell cultures (GH3, AtT-20) and primary pituitary tissue explants to measure peptide release in response to DSIP

This bidirectional relationship is vital for preventing and managing conditions such as irritable bowel syndrome, highlighting the importance of peptides in gut health