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During Phase I and Phase II liver detoxification, glutathione plays a pivotal role in transforming harmful substances into water-soluble compounds that can be easily excreted

Luminescence was recorded for 45 min at 1 min intervals using a Fluoroskan Ascent FL (Thermo Scientific, Rockford, IL, USA) fluorimeter (excitation: 538 nm, emission: 604 nm), and the slopes were used for calculations

The genes encoding DDB1 , CULLIN4A , RBX1 , DET1 , DDA1 , c-Jun , STK40 , ETS2 , Ube2e family ( Ube2e1 ~ 3 ), Ube2d3, Uba3, UBC12, APPBP1 and NEDD8 were amplified from cDNA by PCR and subcloned into pCAG vectors with different tags, respectively

Among these, cholesterol metabolism plays a context-dependent role in ferroptosis, with cholesterol and its intermediates either promoting or suppressing ferroptotic cell death depending on cellular and microenvironmental conditions