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For the Zn-only group, concentrations were adjusted according to the Zn content corresponding to the EGCGZn nanocomplex (EGCG: Zn = 3:1, molar ratio), resulting in Zn concentrations of 4.2, 8.3, 16.7,33.3, 66.7, and 133.3 M

Scaini G, Valvassori SS, Diaz AP, Lima CN, Benevenuto D, Fries GR, et al

After centrifugation, the homogenate was incubated with 200ml of KPBS pre-washed anti-HA magnetic beads (Thermo Scientific Pierce 88837) on a rotator shaker for 5min at 4C