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Before the start, approval was obtained under reference number 1/2022 PEDI 44 from the Institutional Research Board in Menoufia Faculty of Medicine, which works in accordance with the Declaration of Helsinki, and the trial was registered at the Pan African Clinical Trials Registry under number (PACTR202401875833105)
Kwon et al., 2019)
Step 14 Add 50l of Stop Solution to each well
According to data, the daily requirement for selenium is 55 mcg, and one Brazil nut can contain between 68 and 91 mcg
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