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Reconstitution 3-step visual Reconstitute , add bacteriostatic water down the wall of the vial Measure , use the calculator (section 8) to compute the required volume Store , refrigerator 2 to 8 C, protect from light Detailed protocol What you will need: A vial of AOD-9604 (5 mg lyophilizate) 2 to 2.5 ml of bacteriostatic water (contains 0.9 % benzyl alcohol, a preservative that prevents bacterial growth) Insulin syringe 1 ml / 29G Procedure: Allow the vial of AOD-9604 to reach room temperature (15 to 20 min)

The lymph node was located on the basis of Evans Blue staining, immobilized with forceps, and 12 10 4 cells suspended in 1 PBS were injected in a volume of 10 l into the popliteal lymph node using a 27 G Hamilton syringe

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This in turn enables the activation of defense signaling proteins and transcription factors which helps to establish innate immune memory (Lindermayr and Yildirim, 2025)