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2.3 Determination of biochemical indicators 2.3.1 Determination of glutathione levels in heart tissue The measurements of oxidized (GSSG) and reduced glutathione (GSH) were performed in the heart homogenates with Ellmans reagent ( 2.3.2 Measurement of the H 2 S content The content of H 2 S was determined in the suspension of heart mitochondria as described previously with modifications (Strutynska et al., 2022)

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Rates of antimicrobial-related adverse outcomes, Clostridioides difficile infections, and secondary infection with antibiotic-resistant organisms were similar between the seven-day and 14-day groups