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The abnormalities are not specific and can range from depression or mild memory impairment to global dementia

Although dietary fiber has been associated with microbiome changes 75 , this is unlikely to account for the microbiome changes observed herein since fiber content was the same across diets

GLUT4, which is primarily located in striated muscle (skeletal and cardiac) and adipose tissue, plays a major role in regulating glucose uptake and overall energy balance

It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile